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pET28_LmFPPS
(Plasmid #186322)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186322 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FPPS
  • Alt name
    FDPS
  • Alt name
    Farnesyl Diphosphate Synthase
  • Species
    Leishmania major
  • Insert Size (bp)
    1089
  • GenBank ID
    XM_001683235
  • Tag / Fusion Protein
    • His Tag (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also see Maheshwari et al. 2020 "Identifying Structural Determinants of Product Specificity in Leishmania major Farnesyl Diphosphate Synthase". Biochemistry, 59(29), 2751–2759. https://doi.org/10.1021/acs.biochem.0c00432. PMID: 32584028 PMCID: PMC8049779.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28_LmFPPS was a gift from Sandra Gabelli (Addgene plasmid # 186322 ; http://n2t.net/addgene:186322 ; RRID:Addgene_186322)

  • For your References section:

    Structural and thermodynamic basis of the inhibition of Leishmania major farnesyl diphosphate synthase by nitrogen-containing bisphosphonates. Aripirala S, Gonzalez-Pacanowska D, Oldfield E, Kaiser M, Amzel LM, Gabelli SB. Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):802-10. doi: 10.1107/S1399004713033221. Epub 2014 Feb 22. 10.1107/S1399004713033221 PubMed 24598749
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