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Addgene

pQE80_T5_AP10-ehaA_LacI
(Plasmid #186318)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186318 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE80
  • Backbone manufacturer
    Qiagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEBExpress Iq Competent E coli
  • Growth instructions
    Grows in normal cloning strains and conditions. Provided in the NEBExpress Iq strain for ease of expression induction.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP10-ehaA
  • Species
    Synthetic
  • Insert Size (bp)
    1239

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE80_T5_AP10-ehaA_LacI was a gift from George Church (Addgene plasmid # 186318 ; http://n2t.net/addgene:186318 ; RRID:Addgene_186318)

  • For your References section:

    helixCAM: A platform for programmable cellular assembly in bacteria and human cells. Chao G, Wannier TM, Gutierrez C, Borders NC, Appleton E, Chadha A, Lebar T, Church GM. Cell. 2022 Aug 23. pii: S0092-8674(22)01061-3. doi: 10.1016/j.cell.2022.08.012. 10.1016/j.cell.2022.08.012 PubMed 36055250
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