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Addgene

PB_TRE_sg30-PDGFR_EF1a_mCherry
(Plasmid #186309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB
  • Backbone manufacturer
    SBI
  • Modifications to backbone
    mCherry inserted between rtTA and puromycin resistance gene
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    sg30-PDGFR
  • Species
    Synthetic
  • Insert Size (bp)
    396
  • Tag / Fusion Protein
    • His, myc

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    714

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB_TRE_sg30-PDGFR_EF1a_mCherry was a gift from George Church (Addgene plasmid # 186309 ; http://n2t.net/addgene:186309 ; RRID:Addgene_186309)

  • For your References section:

    helixCAM: A platform for programmable cellular assembly in bacteria and human cells. Chao G, Wannier TM, Gutierrez C, Borders NC, Appleton E, Chadha A, Lebar T, Church GM. Cell. 2022 Aug 23. pii: S0092-8674(22)01061-3. doi: 10.1016/j.cell.2022.08.012. 10.1016/j.cell.2022.08.012 PubMed 36055250
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