PB_TRE_Z17-PDGFR_EF1a_mCherry
(Plasmid
#186303)
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PurposePlasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186303 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB
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Backbone manufacturerSBI
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Modifications to backbonemCherry inserted between rtTA and puromycin resistance gene
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameZ17-PDGFR
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SpeciesSynthetic
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Insert Size (bp)416
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Tag
/ Fusion Protein
- HA, myc
Gene/Insert 2
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Gene/Insert namemCherry
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SpeciesSynthetic
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Insert Size (bp)714
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB_TRE_Z17-PDGFR_EF1a_mCherry was a gift from George Church (Addgene plasmid # 186303 ; http://n2t.net/addgene:186303 ; RRID:Addgene_186303) -
For your References section:
helixCAM: A platform for programmable cellular assembly in bacteria and human cells. Chao G, Wannier TM, Gutierrez C, Borders NC, Appleton E, Chadha A, Lebar T, Church GM. Cell. 2022 Aug 23. pii: S0092-8674(22)01061-3. doi: 10.1016/j.cell.2022.08.012. 10.1016/j.cell.2022.08.012 PubMed 36055250