Integrin Beta1-mPA-GFP
(Plasmid #186302)

• Purpose: Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter
• Depositing Lab: Pakorn Kanchanawong
• Publication: Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s41596-022-00717-3. doi: 10.1038/s41596-022-00717-3.

Backbone

• Vector backbone: Clontech N1
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 7213
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Integrin Beta 1
• Alt name: ITGB1
• Alt name: CD29
• Species: H. sapiens (human)
• Insert Size (bp): 2394
• GenBank ID: NM_002211.3
• Entrez Gene: ITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)
• Promoter: CMV
• Tag / Fusion Protein:
  • mPA-GFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Photoconvertable:
On: Excitation 504 nm, Emission 517 nm
Off to On: 400 nm