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PET28a-cpLumiLuc-amylase sensor
(Plasmid #186293)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186293 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PET28a
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6542
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    cpLumiLuc-amylase sensor
  • Insert Size (bp)
    1302
  • Tag / Fusion Protein
    • HisTag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PET28a-cpLumiLuc-amylase sensor was a gift from Kirill Alexandrov (Addgene plasmid # 186293 ; http://n2t.net/addgene:186293 ; RRID:Addgene_186293)

  • For your References section:

    Engineering and exploiting synthetic allostery of NanoLuc luciferase. Guo Z, Parakra RD, Xiong Y, Johnston WA, Walden P, Edwardraja S, Moradi SV, Ungerer JPJ, Ai HW, Phillips JJ, Alexandrov K. Nat Commun. 2022 Feb 10;13(1):789. doi: 10.1038/s41467-022-28425-2. 10.1038/s41467-022-28425-2 PubMed 35145068
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