pCRISPR_full-site
(Plasmid
#186277)
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PurposeReporter plasmid for full-site selection assay
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186277 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2279
- Total vector size (bp) 3342
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameThiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
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Insert Size (bp)1063
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRISPR_full-site was a gift from Jennifer Doudna (Addgene plasmid # 186277 ; http://n2t.net/addgene:186277 ; RRID:Addgene_186277) -
For your References section:
Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex. Wang JY, Hoel CM, Al-Shayeb B, Banfield JF, Brohawn SG, Doudna JA. Nat Commun. 2021 May 6;12(1):2571. doi: 10.1038/s41467-021-22900-y. 10.1038/s41467-021-22900-y PubMed 33958590