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pET_His6-MBP-TEV-Cas6-RT-Cas1_WT
(Plasmid #186274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186274 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET His6 MBP N10 TEV LIC cloning vector (2C-T)
  • Backbone size w/o insert (bp) 5953
  • Total vector size (bp) 8899
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Thiomicropira Cas6-RT-Cas1
  • Insert Size (bp)
    2946
  • Tag / Fusion Protein
    • His6-MBP (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET_His6-MBP-TEV-Cas6-RT-Cas1_WT was a gift from Jennifer Doudna (Addgene plasmid # 186274 ; http://n2t.net/addgene:186274 ; RRID:Addgene_186274)

  • For your References section:

    Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex. Wang JY, Hoel CM, Al-Shayeb B, Banfield JF, Brohawn SG, Doudna JA. Nat Commun. 2021 May 6;12(1):2571. doi: 10.1038/s41467-021-22900-y. 10.1038/s41467-021-22900-y PubMed 33958590
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