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Addgene

pAP01
(Plasmid #186260)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186260 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA531
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • Promoter PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTATAGTCGAATAAA)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGAAAGGCCCAGTCTTTC
  • 3′ sequencing primer CGCCAGGGTTTTCCCAGTCACGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAP01 was a gift from Yongku Cho (Addgene plasmid # 186260 ; http://n2t.net/addgene:186260 ; RRID:Addgene_186260)

  • For your References section:

    Optogenetics in Sinorhizobium meliloti Enables Spatial Control of Exopolysaccharide Production and Biofilm Structure. Pirhanov A, Bridges CM, Goodwin RA, Guo YS, Furrer J, Shor LM, Gage DJ, Cho YK. ACS Synth Biol. 2021 Feb 19;10(2):345-356. doi: 10.1021/acssynbio.0c00498. Epub 2021 Jan 19. 10.1021/acssynbio.0c00498 PubMed 33465305
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