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Addgene

pET3a-FruK
(Plasmid #186256)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 5500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    1-phosphofructokinase
  • Alt name
    FruK
  • Alt name
    Fructose-1-Phosphate Kinase
  • Species
    E. coli
  • Insert Size (bp)
    1000
  • Entrez Gene
    fruK (a.k.a. b2168, ECK2161, fpk)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GATGCGTCCGGCGTAG
  • 3′ sequencing primer GCTCAGCGGTGGCAGC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Gift from Dr. van Schaftingen to Dr. Swint-Kruse at KUMC
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3a-FruK was a gift from Liskin Swint-Kruse & Emile Van Schaftingen (Addgene plasmid # 186256 ; http://n2t.net/addgene:186256 ; RRID:Addgene_186256)

  • For your References section:

    Overexpression and purification of fructose-1-phosphate kinase from Escherichia coli: application to the assay of fructose 1-phosphate. Veiga-da-Cunha M, Hoyoux A, Van Schaftingen E. Protein Expr Purif. 2000 Jun;19(1):48-52. doi: 10.1006/prep.2000.1237. 10.1006/prep.2000.1237 PubMed 10833389
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