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Addgene

pBA630
(Plasmid #186241)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186241 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pTet-RfxCas13d + crRNA negative control
  • gRNA/shRNA sequence
    Unspecified
  • Species
    Other

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA630 was a gift from Jennifer Doudna (Addgene plasmid # 186241 ; http://n2t.net/addgene:186241 ; RRID:Addgene_186241)

  • For your References section:

    Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing. Adler BA, Hessler T, Cress BF, Lahiri A, Mutalik VK, Barrangou R, Banfield J, Doudna JA. Nat Microbiol. 2022 Dec;7(12):1967-1979. doi: 10.1038/s41564-022-01258-x. Epub 2022 Oct 31. 10.1038/s41564-022-01258-x PubMed 36316451
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