pCS2-SP-FKBP-mCherry-TM-V5
(Plasmid
#186226)
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PurposemRNA synthesis of rapalog-induced cell adhesion molecule
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSP-FKBP-mCherry-TM-V5
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SpeciesSynthetic
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Insert Size (bp)1317
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ATTTAGGTGACACTATAGA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-SP-FKBP-mCherry-TM-V5 was a gift from Yoshihiro Morishita (Addgene plasmid # 186226 ; http://n2t.net/addgene:186226 ; RRID:Addgene_186226) -
For your References section:
Rapalog-induced cell adhesion molecule inhibits mesoderm migration in Xenopus embryos by increasing frequency of adhesion to the ectoderm. Usami C, Inomata H. Genes Cells. 2022 Jun;27(6):436-450. doi: 10.1111/gtc.12937. Epub 2022 May 4. 10.1111/gtc.12937 PubMed 35437867