pBMN BFP-Parkin
(Plasmid #186221)

• Purpose: For expression of Parkin tagged with BFP
• Depositing Lab: Michael Lazarou
• Publication: Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021.

Backbone

• Vector backbone: pBMN
• Total vector size (bp): 7094
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Parkin
• Alt name: PARK2
• Alt name: PRKN
• Alt name: LPRS2
• Species: H. sapiens (human)
• Insert Size (bp): 1395
• GenBank ID: NM_004562.3
• Entrez Gene: PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
• Tag / Fusion Protein:
  • BFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pBMN 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry