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mitoTALECD for_ -13G_1397CN
(Plasmid #186199)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186199 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pK7WG2
  • Backbone manufacturer
    VIB, Ghent University
  • Total vector size (bp) 2426
  • Modifications to backbone
    RPS5A promoter, Oleosin-GFP marker added in between Left and right border of T-DNA.
  • Vector type
    Synthetic Biology ; Ti plasmid for plant transformation
  • Selectable markers
    Kanamycin and seed GFP fluorescence by OleosinGFP for plant transformation

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
  • Alt name
    for specific base editing of -13th G to A of the RNA editing site C of ATP1 by OTP87
  • Species
    Synthetic
  • Insert Size (bp)
    2967
  • GenBank ID

Gene/Insert 2

  • Gene/Insert name
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
  • Alt name
    for specific base editing of -13th G to A of the RNA editing site C of ATP1 by OTP87
  • Insert Size (bp)
    3411

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mitoTALECD for_ -13G_1397CN was a gift from Shin-ichi Arimura (Addgene plasmid # 186199 ; http://n2t.net/addgene:186199 ; RRID:Addgene_186199)

  • For your References section:

    Targeted base editing in the mitochondrial genome of Arabidopsis thaliana. Nakazato I, Okuno M, Zhou C, Itoh T, Tsutsumi N, Takenaka M, Arimura SI. Proc Natl Acad Sci U S A. 2022 May 17;119(20):e2121177119. doi: 10.1073/pnas.2121177119. Epub 2022 May 13. 10.1073/pnas.2121177119 PubMed 35561225
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