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Addgene

pAAV-TRE-Tight2-AkaLuc
(Plasmid #186186)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186186 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW3SL
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AkaLuc luciferase
  • Alt name
    firefly luciferase mutant protein
  • Species
    Synthetic
  • Promoter TRE-Tight2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TRE-Tight2-AkaLuc was a gift from Vladislav Verkhusha (Addgene plasmid # 186186 ; http://n2t.net/addgene:186186 ; RRID:Addgene_186186)

  • For your References section:

    Optogenetic manipulation and photoacoustic imaging using a near-infrared transgenic mouse model. Kasatkina LA, Ma C, Matlashov ME, Vu T, Li M, Kaberniuk AA, Yao J, Verkhusha VV. Nat Commun. 2022 May 19;13(1):2813. doi: 10.1038/s41467-022-30547-6. 10.1038/s41467-022-30547-6 PubMed 35589810
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