p166
(Plasmid
#186180)
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PurposeShotgun Metagenomic Spike-in
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186180 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57
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Backbone manufacturerGenScript
- Backbone size w/o insert (bp) 2710
- Total vector size (bp) 4709
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Vector typeSynthetic Biology ; Cloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesynDNA 166
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Alt namep166
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SpeciesSynthetic
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Insert Size (bp)2000
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GTAAAACGACGGCCAGTG
- 3′ sequencing primer GGAAACAGCTATGACCATG (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe synthetic gene was manufactured by GenScript.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p166 was a gift from Karsten Zengler (Addgene plasmid # 186180 ; http://n2t.net/addgene:186180 ; RRID:Addgene_186180) -
For your References section:
synDNA-a Synthetic DNA Spike-in Method for Absolute Quantification of Shotgun Metagenomic Sequencing. Zaramela LS, Tjuanta M, Moyne O, Neal M, Zengler K. mSystems. 2022 Dec 20;7(6):e0044722. doi: 10.1128/msystems.00447-22. Epub 2022 Nov 1. 10.1128/msystems.00447-22 PubMed 36317886
Map uploaded by the depositor.
