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Addgene

p156
(Plasmid #186179)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186179 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    GenScript
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 4710
  • Vector type
    Synthetic Biology, Unspecified ; Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synDNA 156
  • Alt name
    p156
  • Species
    Synthetic
  • Insert Size (bp)
    2000

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAGTG
  • 3′ sequencing primer GGAAACAGCTATGACCATG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The synthetic gene was manufactured by GenScript.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p156 was a gift from Karsten Zengler (Addgene plasmid # 186179 ; http://n2t.net/addgene:186179 ; RRID:Addgene_186179)

  • For your References section:

    synDNA-a Synthetic DNA Spike-in Method for Absolute Quantification of Shotgun Metagenomic Sequencing. Zaramela LS, Tjuanta M, Moyne O, Neal M, Zengler K. mSystems. 2022 Dec 20;7(6):e0044722. doi: 10.1128/msystems.00447-22. Epub 2022 Nov 1. 10.1128/msystems.00447-22 PubMed 36317886
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