pKI-cjOSR1-EGFP-PGK-Puro
(Plasmid
#186170)
-
PurposeKnock-in gene targeting in marmoset cells at OSR1 locus
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 186170 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCR-Blunt II-TOPO
-
Backbone manufacturerThermo Fisher Scientific
-
Vector typeMarmoset targeting
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEGFP
-
SpeciesAequorea victoria
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pKI-cjOSR1-EGFP-PGK-Puro was a gift from Sho Yoshimatsu (Addgene plasmid # 186170 ; http://n2t.net/addgene:186170 ; RRID:Addgene_186170) -
For your References section:
A New Horizon in Reproductive Research with Pluripotent Stem Cells: Successful In Vitro Gametogenesis in Rodents, Its Application to Large Animals, and Future In Vitro Reconstitution of Reproductive Organs Such as "Uteroid" and "Oviductoid". Yoshimatsu S, Kisu I, Qian E, Noce T. Biology (Basel). 2022 Jun 29;11(7):987. doi: 10.3390/biology11070987. 10.3390/biology11070987 PubMed 36101367