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Addgene

p130Cas Y15F
(Plasmid #186154)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac Htb
  • Backbone size w/o insert (bp) 4858
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BCAR1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2672
  • Mutation
    15Y residues mutated to Alanine
  • Entrez Gene
    Bcar1 (a.k.a. Cas, Crkas, P130CAS)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • His6 (Tev) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer polyhedrin-fwd (5-AAATGATAACCATCTCGC-3)
  • 3′ sequencing primer SV40pA-R (5-GAAATTTGTGATGCTATTGC-3)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthesized

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p130Cas Y15F was a gift from Michael Rosen (Addgene plasmid # 186154 ; http://n2t.net/addgene:186154 ; RRID:Addgene_186154)

  • For your References section:

    Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation. Case LB, De Pasquale M, Henry L, Rosen MK. Elife. 2022 Jan 20;11. pii: 72588. doi: 10.7554/eLife.72588. 10.7554/eLife.72588 PubMed 35049497
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