GFP-Paxillin DN
(Plasmid
#186153)
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PurposeFor expressing paxillin variants in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186153 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemEGFP-C1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePXN
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SpeciesH. sapiens (human)
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Insert Size (bp)2634
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MutationPaxilln residues 1-321 added to N-terminus of WT Paxillin
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Entrez GenePXN
- Promoter cmv
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Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EGFP-C-For (5-CATGGTCCTGCTGGAGTTCGTG-3)
- 3′ sequencing primer SV40pA-R (5-GAAATTTGTGATGCTATTGC-3) (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymEGFP-C1 (Addgene Plasmid #54759
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-Paxillin DN was a gift from Michael Rosen (Addgene plasmid # 186153 ; http://n2t.net/addgene:186153 ; RRID:Addgene_186153) -
For your References section:
Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation. Case LB, De Pasquale M, Henry L, Rosen MK. Elife. 2022 Jan 20;11. pii: 72588. doi: 10.7554/eLife.72588. 10.7554/eLife.72588 PubMed 35049497
