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Addgene

Nephrin
(Plasmid #186134)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186134 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMal-RTH
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NPHS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    219
  • Mutation
    Amino acid residues 1174-1223, with mutations: Y1183F, Y1210F
  • Entrez Gene
    NPHS1 (a.k.a. CNF, NPHN, nephrin)
  • Promoter Lac
  • Tags / Fusion Proteins
    • MBP (N terminal on insert)
    • His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pMAL-Fwd
  • 3′ sequencing primer pMAL-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nephrin was a gift from Michael Rosen (Addgene plasmid # 186134 ; http://n2t.net/addgene:186134 ; RRID:Addgene_186134)

  • For your References section:

    Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation. Case LB, De Pasquale M, Henry L, Rosen MK. Elife. 2022 Jan 20;11. pii: 72588. doi: 10.7554/eLife.72588. 10.7554/eLife.72588 PubMed 35049497
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