Nck
(Plasmid
#186133)
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PurposeFor expression of recombinant Nck in bacteria cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186133 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX2T
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNCK1
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SpeciesH. sapiens (human)
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Insert Size (bp)1137
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MutationAmino acid residues 1-377, with mutations: C139S, C232A, C266S, C340S
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Entrez GeneNCK1 (a.k.a. NCK, NCKalpha, nck-1)
- Promoter Lac
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pGEX-5'
- 3′ sequencing primer pGEX-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nck was a gift from Michael Rosen (Addgene plasmid # 186133 ; http://n2t.net/addgene:186133 ; RRID:Addgene_186133) -
For your References section:
Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation. Case LB, De Pasquale M, Henry L, Rosen MK. Elife. 2022 Jan 20;11. pii: 72588. doi: 10.7554/eLife.72588. 10.7554/eLife.72588 PubMed 35049497