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Addgene

pTR-452 tNGFR-P2A-Lck
(Plasmid #186080)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186080 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PUC19
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LCK
  • Species
    H. sapiens (human)
  • Mutation
    WT with tNGFR sequence
  • Entrez Gene
    LCK (a.k.a. IMD22, LSK, YT16, p56lck, pp58lck)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTR-452 tNGFR-P2A-Lck was a gift from Alexander Marson (Addgene plasmid # 186080 ; http://n2t.net/addgene:186080 ; RRID:Addgene_186080)

  • For your References section:

    High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails. Shy BR, Vykunta VS, Ha A, Talbot A, Roth TL, Nguyen DN, Pfeifer WG, Chen YY, Blaeschke F, Shifrut E, Vedova S, Mamedov MR, Chung JJ, Li H, Yu R, Wu D, Wolf J, Martin TG, Castro CE, Ye L, Esensten JH, Eyquem J, Marson A. Nat Biotechnol. 2022 Aug 25:10.1038/s41587-022-01418-8. doi: 10.1038/s41587-022-01418-8. 10.1038/s41587-022-01418-8 PubMed 36008610
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