AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE
(Plasmid
#186047)
-
PurposeAAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expression
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 186047 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneAAV
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameLifeAct-jGCaMP8f
-
SpeciesSynthetic
-
Insert Size (bp)1320
- Promoter CAG
-
Tag
/ Fusion Protein
- LifeAct-6xHis (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE was a gift from Loren Looger (Addgene plasmid # 186047 ; http://n2t.net/addgene:186047 ; RRID:Addgene_186047)