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Addgene

pHEN2 F8-2
(Plasmid #186033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHEN2
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 4981
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Supplement media with 0.5% glucose
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VHH F8-2
  • Species
    Synthetic
  • Insert Size (bp)
    389
  • Promoter lac
  • Tags / Fusion Proteins
    • 6His (C terminal on backbone)
    • Myc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer M13rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHEN2 F8-2 was a gift from Isabelle Landrieu (Addgene plasmid # 186033 ; http://n2t.net/addgene:186033 ; RRID:Addgene_186033)

  • For your References section:

    Inhibition of Tau seeding by targeting Tau nucleation core within neurons with a single domain antibody fragment. Danis C, Dupre E, Zejneli O, Caillierez R, Arrial A, Begard S, Mortelecque J, Eddarkaoui S, Loyens A, Cantrelle FX, Hanoulle X, Rain JC, Colin M, Buee L, Landrieu I. Mol Ther. 2022 Apr 6;30(4):1484-1499. doi: 10.1016/j.ymthe.2022.01.009. Epub 2022 Jan 7. 10.1016/j.ymthe.2022.01.009 PubMed 35007758
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