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Addgene

pOP005
(Plasmid #186030)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186030 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOA-RQ
  • Backbone manufacturer
    GeneArt (Thermo Fisher Scientific)
  • Backbone size w/o insert (bp) 2414
  • Total vector size (bp) 3252
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
  • Species
    Synthetic
  • Insert Size (bp)
    838

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer GAAACAGCTATGACCATGTTAATGCAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOP005 was a gift from Georgi Belogurov (Addgene plasmid # 186030 ; http://n2t.net/addgene:186030 ; RRID:Addgene_186030)

  • For your References section:

    Fluorogenic RNA aptamers to probe transcription initiation and co-transcriptional RNA folding by multi-subunit RNA polymerases. Huang YH, Trapp V, Puro O, Makinen JJ, Metsa-Ketela M, Wahl MC, Belogurov GA. Methods Enzymol. 2022;675:207-233. doi: 10.1016/bs.mie.2022.07.010. Epub 2022 Aug 19. 10.1016/bs.mie.2022.07.010 PubMed 36220271
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