myc-Rictor
(Plasmid #1860)

• Purpose: Please see Addgene item #11367 for an updated version of this plasmid.
• Depositing Lab: David Sabatini
• Publication: Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Backbone

• Vector backbone: pRK-5
• Backbone manufacturer: BD Pharmingen
• Backbone size w/o insert (bp): 4754
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rictor
• Species: H. sapiens (human)
• Entrez Gene: RICTOR (a.k.a. AVO3, PIA, hAVO3)
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: This plasmid contains 2 amino acids that are different than the published NCBI sequence. The changes can be viewed in the Addgene Full Sequence link above- T->C at bp 1312 resulting in amino acid 35 being changed from S->P and a T->C change at bp 1556 resulting in amino acid 116 being changed from I->T. A new version of this plasmid with the corrected sequence is available (Addgene #11367).