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Addgene

ORF3a-1x-BioID2 pBabe-puro (SARS-CoV-2)
(Plasmid #185989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 ORF3a
  • Species
    SARS-CoV-2
  • Entrez Gene
    ORF3a (a.k.a. GU280_gp03)
  • Promoter LTR
  • Tags / Fusion Proteins
    • BioID2 (C terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBabeF
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ORF3a-1x-BioID2 pBabe-puro (SARS-CoV-2) was a gift from Kyle Roux (Addgene plasmid # 185989 ; http://n2t.net/addgene:185989 ; RRID:Addgene_185989)

  • For your References section:

    A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins. May DG, Martin-Sancho L, Anschau V, Liu S, Chrisopulos RJ, Scott KL, Halfmann CT, Diaz Pena R, Pratt D, Campos AR, Roux KJ. Viruses. 2022 Mar 15;14(3). pii: v14030611. doi: 10.3390/v14030611. 10.3390/v14030611 PubMed 35337019
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