pKIF1C-mCherry-FRB
(Plasmid
#185982)
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PurposeMammalian expression of human KIF1C with mCherry and FRB tags.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185982 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepbactin-GFP-FRB
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKIF1C
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Alt nameSAX2
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Alt nameKIAA0706
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Alt nameLTXS1
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SpeciesH. sapiens (human)
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Entrez GeneKIF1C (a.k.a. LTXS1, SATX2, SAX2, SPAX2, SPG58)
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Tag
/ Fusion Protein
- mCherry and FRB (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKIF1C-mCherry-FRB was a gift from Anne Straube (Addgene plasmid # 185982 ; http://n2t.net/addgene:185982 ; RRID:Addgene_185982) -
For your References section:
Force generation of KIF1C is impaired by pathogenic mutations. Siddiqui N, Roth D, Toleikis A, Zwetsloot AJ, Cross RA, Straube A. Curr Biol. 2022 Sep 12;32(17):3862-3870.e6. doi: 10.1016/j.cub.2022.07.029. Epub 2022 Aug 11. 10.1016/j.cub.2022.07.029 PubMed 35961316