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T_antiEGFPsynsrRNA_UA
(Plasmid #185974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Pz
  • Backbone manufacturer
    expressys.
  • Backbone size w/o insert (bp) 2112
  • Total vector size (bp) 2548
  • Modifications to backbone
    Insertion of PlTetO-1antiEGFPsynsrRNA
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PlTetO-1 AntiEGFPsynsrRNA
  • Species
    Synthetic
  • Insert Size (bp)
    436
  • Promoter PlTetO-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AatII (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer GAGGCCCTTTCGTCTTCAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T_antiEGFPsynsrRNA_UA was a gift from Sangram Bagh (Addgene plasmid # 185974 ; http://n2t.net/addgene:185974 ; RRID:Addgene_185974)

  • For your References section:

    A microgravity responsive synthetic genetic device in Escherichia coli. Mukhopadhyay S, Bagh S. Biosens Bioelectron. 2020 Nov 1;167:112462. doi: 10.1016/j.bios.2020.112462. Epub 2020 Aug 1. 10.1016/j.bios.2020.112462 PubMed 32781386
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