Skip to main content
Addgene

pB6-Rosa26-cre_dre_reporter_1_2
(Plasmid #185966)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Custom
  • Backbone manufacturer
    Hyung-song Nam, Capecchi laboratory
  • Vector type
    Mammalian Expression, Mouse Targeting, Cre/Lox ; Dre/Rox
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sfGFP, tdTomato
  • Species
    Synthetic

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    FNF is from plasmid #22687. CAG promoter is from pCAGGS obtained from BCCM/LMBP. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland. SV40 pA is from Clontech. EMCV IRES is from Peter Mombaerts. tdTomato is from Roger Tsien.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cre → sfGFP expression. dre → tdTomato expression.

Expression of sfGFP and tdTomato are independent of each other. "OR."

Actual mouse was not generated.

The EMCV IRES fragment from Dr. Peter Mombaerts is the same EMCV IRES used in the Id1-IRES-creERT2 knock-in mouse line (http://www.informatics.jax.org/allele/MGI:4366863)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB6-Rosa26-cre_dre_reporter_1_2 was a gift from Mario Capecchi (Addgene plasmid # 185966 ; http://n2t.net/addgene:185966 ; RRID:Addgene_185966)
Related items: