pB6-Rosa26-cre_dre_reporter_1_1a
(Plasmid
#185964)
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PurposeB6J Rosa26-cre dre reporter 1.1a allele targeting vector, low copy number
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185964 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCustom
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Backbone manufacturerHyung-song Nam, Capecchi laboratory
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Vector typeMammalian Expression, Mouse Targeting, Cre/Lox ; Dre/Rox
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesfGFP, tdTomato
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byFNF is from plasmid #22687. CAG promoter is from pCAGGS obtained from BCCM/LMBP. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland. SV40 pA is from Clontech. tdTomato is from Roger Tsien.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EGFP in pB6-Rosa26-cre_dre_reporter_1_1 replaced with sfGFP.
cre → sfGFP expression. dre → tdTomato expression.
Expression of sfGFP and tdTomato are independent of each other. "OR."
Actual mouse was not generated, hence unknown whether this construct would result in the same "phenotype' as the pB6-Rosa26-cre_dre_reporter_1_1 mouse.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB6-Rosa26-cre_dre_reporter_1_1a was a gift from Mario Capecchi (Addgene plasmid # 185964 ; http://n2t.net/addgene:185964 ; RRID:Addgene_185964)