pB6-Rosa26-cre_dre_reporter_1_1
(Plasmid #185963)

• Purpose: B6J Rosa26-cre dre reporter 1.1 allele targeting vector, low copy number
• Depositing Lab: Mario Capecchi
• Publication: Miscellaneous gene targeting vector plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Mouse Targeting, Cre/Lox ; Dre/Rox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EGFP, tdTomato
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • pROSA26-DTA-cre dre reporter 1.1.gb
• A portion of this plasmid was derived from a plasmid made by: FNF is from plasmid #22687. CAG promoter is from pCAGGS obtained from BCCM/LMBP. EGFP is from Clontech. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland. SV40 pA is from Clontech. tdTomato is from Roger Tsien.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cre → EGFP expression. dre → tdTomato expression.

Expression of EGFP and tdTomato are independent of each other. "OR."

The actual mouse had a "phenotype" - the males and females were fine otherwise, but the females did not nurse the pups.