pMRE-Himar-134
(Plasmid
#185925)
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PurposeHimar transposon delivery plasmid that delivers a orange fluorescent mOrange2 protein gene
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnspecified
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)S17-1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemOrange2
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.03.26.437257v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRE-Himar-134 was a gift from Mitja Remus-Emsermann (Addgene plasmid # 185925 ; http://n2t.net/addgene:185925 ; RRID:Addgene_185925) -
For your References section:
Chromatic Bacteria v.2-A Himar1 Transposon-Based Delivery Vector to Extend the Host Range of a Toolbox to Fluorescently Tag Bacteria. Stocks C, Schlechter R, Remus-Emsermann M. Bacteria 2022, 1(1), 56-65 10.3390/bacteria1010006