pMP190
(Plasmid #185922)

• Purpose: (Empty Backbone) Broad host range IncQ promoter probe using lacZ
• Depositing Lab: Herman Spaink
• Publication: Spaink et al Plant Mol Biol. 1987 Jan;9(1):27-39. doi: 10.1007/BF00017984.

Backbone

• Vector backbone: pMP184
• Backbone manufacturer: Spaink
• Backbone size (bp): 15000
• Modifications to backbone: Insertion of polylinker
• Vector type: Bacterial Expression ; IncQ
• Promoter: none
• Tag / Fusion Protein:
  • LacZ

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): KMBL1164
• Copy number: Unknown

Cloning Information

• 5′ sequencing primer: Not Known
• 3′ sequencing primer: Not Known

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Polylinker is obtained from pIC20H: Marsh JL, Erfle M, Wykes E J: The plC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481-485 (1984).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid also confers streptomycin resistance.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.