pMP220
(Plasmid
#185921)
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Purpose(Empty Backbone) Broad host range IncP promoter probe using lacZ
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185921 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMP103
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Backbone manufacturerSpaink
- Backbone size (bp) 10400
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Modifications to backbonePolylinker
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Vector typeBacterial Expression ; IncP
- Promoter None
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Tag
/ Fusion Protein
- LacZ
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- 5′ sequencing primer Not known
- 3′ sequencing primer Not known (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe polylinker was from pIC20H Marsh JL, Erfle M, Wykes E J: The plC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481-485 (1984)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
the polylinker of pIC20H is broadly available and isn't covered by an MTA
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMP220 was a gift from Herman Spaink (Addgene plasmid # 185921 ; http://n2t.net/addgene:185921 ; RRID:Addgene_185921) -
For your References section:
Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Spaink HP, Okker RJ, Wijffelman CA, Pees E, Lugtenberg BJ. Plant Mol Biol. 1987 Jan;9(1):27-39. doi: 10.1007/BF00017984. 10.1007/BF00017984 PubMed 24276795
Map uploaded by the depositor.
