p323.hSyn.Halo-mFMRP.Wpre
(Plasmid #185909)

• Purpose: Expresses FMRP fused to HaloTag for protein localization through fluorescence imaging
• Depositing Lab: Young J Yoon
• Publication: Monday et al Neuron. 2022 Aug 17;110(16):2588-2606.e6. doi: 10.1016/j.neuron.2022.05.024. Epub 2022 Jun 20.

Backbone

• Vector backbone: Lentiviral vector (Gen 3)
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 9800
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HaloTag fused to mouse Fragile X mental retardation protein
• Alt name: Halo-FMRP
• Alt name: Halo-mFMRP
• Species: M. musculus (mouse)
• Insert Size (bp): 2800
• Entrez Gene: Ctsa (a.k.a. PPCA, Ppgb)
• Promoter: hSyn (human Synapsin promoter)
• Tag / Fusion Protein:
  • HaloTag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: hSyn promoter primer
• 3′ sequencing primer: Wpre reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry