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Addgene

pfa6a PhoCl-TsCC(B)::KANMX
(Plasmid #185906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pfa6a::KAN
  • Total vector size (bp) 5841
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PhoCl-TsCC(B)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1659
  • Promoter SP6
  • Tag / Fusion Protein
    • PhoCl fluorophore with C-terminal TsCC(B) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pfa6a PhoCl-TsCC(B)::KANMX was a gift from Matthew Good (Addgene plasmid # 185906 ; http://n2t.net/addgene:185906 ; RRID:Addgene_185906)

  • For your References section:

    Designer membraneless organelles sequester native factors for control of cell behavior. Garabedian MV, Wang W, Dabdoub JB, Tong M, Caldwell RM, Benman W, Schuster BS, Deiters A, Good MC. Nat Chem Biol. 2021 Sep;17(9):998-1007. doi: 10.1038/s41589-021-00840-4. Epub 2021 Aug 2. 10.1038/s41589-021-00840-4 PubMed 34341589
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