pIT6EG7ml
(Plasmid
#185886)
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PurposeIn vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (14 copy).
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185886 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepUC19
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesee comments
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SpeciesSynthetic
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MutationERG20 F96W N127W
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PSk.GAL2>Y.FAST-EVBR1.2A-Ec.MBP-Linker-Ll.LS-6*G-ERG20F96W N127W>TRPL41B-PPDA1> RPL25(partial; Arm2)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIT6EG7ml was a gift from Claudia Vickers (Addgene plasmid # 185886 ; http://n2t.net/addgene:185886 ; RRID:Addgene_185886)