pIT6EG7m
(Plasmid
#185885)
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Purpose(Empty Backbone) A backbone plasmid for in vivo gene amplification (HamAmp) of a monoterpene synthetic module (+ GPP synthase without a monoterpene synthase) at RPL25 locus (14 copy)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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MutationNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PSk.GAL2>Y.FAST-EVBR1.2A-Ec.MBP-Linker~SacI~6*G-ERG20F96W N127W>TRPL3 -PPDA1> RPL25(partial; Arm2)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIT6EG7m was a gift from Claudia Vickers (Addgene plasmid # 185885 ; http://n2t.net/addgene:185885 ; RRID:Addgene_185885)