pJCKan
(Plasmid
#185862)
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PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepML104
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
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SpeciesSynthetic
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MutationNone
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJCKan was a gift from Claudia Vickers (Addgene plasmid # 185862 ; http://n2t.net/addgene:185862 ; RRID:Addgene_185862)