pILGFP9E3
(Plasmid
#185842)
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PurposeTesting the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185842 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
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SpeciesSynthetic
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MutationNone
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pILGFP9E3 was a gift from Claudia Vickers (Addgene plasmid # 185842 ; http://n2t.net/addgene:185842 ; RRID:Addgene_185842) -
For your References section:
Engineering eukaryote-like regulatory circuits to expand artificial control mechanisms for metabolic engineering in Saccharomyces cerevisiae. Peng B, Bandari NC, Lu Z, Howard CB, Scott C, Trau M, Dumsday G, Vickers CE. Commun Biol. 2022 Feb 16;5(1):135. doi: 10.1038/s42003-022-03070-z. 10.1038/s42003-022-03070-z PubMed 35173283