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Addgene

pAF7E1
(Plasmid #185839)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185839 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS414
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHAC1>Zif268>MED15>PABF1
  • Species
    Synthetic
  • Mutation
    None

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAF7E1 was a gift from Claudia Vickers (Addgene plasmid # 185839 ; http://n2t.net/addgene:185839 ; RRID:Addgene_185839)

  • For your References section:

    Engineering eukaryote-like regulatory circuits to expand artificial control mechanisms for metabolic engineering in Saccharomyces cerevisiae. Peng B, Bandari NC, Lu Z, Howard CB, Scott C, Trau M, Dumsday G, Vickers CE. Commun Biol. 2022 Feb 16;5(1):135. doi: 10.1038/s42003-022-03070-z. 10.1038/s42003-022-03070-z PubMed 35173283
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