pBS Coupler 1
(Plasmid
#185824)
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PurposeCloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185824 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBlueScript
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 3045
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name(GlyGlyGlyGlySer)x3 linker
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS Coupler 1 was a gift from Atsushi Miyawaki (Addgene plasmid # 185824 ; http://n2t.net/addgene:185824 ; RRID:Addgene_185824) -
For your References section:
Engineering FRET constructs using CFP and YFP. Shimozono S, Miyawaki A. Methods Cell Biol. 2008;85:381-93. doi: 10.1016/S0091-679X(08)85016-9. 10.1016/S0091-679X(08)85016-9 PubMed 18155471
Map uploaded by the depositor.
