6xABRE_A
(Plasmid
#185803)
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PurposeA synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the ABI1 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185803 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSE7
- Total vector size (bp) 7635
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name6xABRE(ABI1)+-86MP+erGFP
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byDr Lina Duan, Dinneny Lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6xABRE_A was a gift from Jose Dinneny (Addgene plasmid # 185803 ; http://n2t.net/addgene:185803 ; RRID:Addgene_185803) -
For your References section:
The 6xABRE Synthetic Promoter Enables the Spatiotemporal Analysis of ABA-Mediated Transcriptional Regulation. Wu R, Duan L, Pruneda-Paz JL, Oh DH, Pound M, Kay S, Dinneny JR. Plant Physiol. 2018 Aug;177(4):1650-1665. doi: 10.1104/pp.18.00401. Epub 2018 Jun 8. 10.1104/pp.18.00401 PubMed 29884679