cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)
(Plasmid
#185800)
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Purposeone plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHAGE-UbiC
- Total vector size (bp) 9777
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecmv-sfGFP-GB1-scAB--ubc-nls-pcp-5xSunTag
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SpeciesSynthetic
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CGTTACATAACTTACGG
- 3′ sequencing primer GTTTACCGCGGTGCCTGA (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P) was a gift from Robin Lee (Addgene plasmid # 185800 ; http://n2t.net/addgene:185800 ; RRID:Addgene_185800) -
For your References section:
Long-term imaging of individual mRNA molecules in living cells. Guo Y, Lee REC. Cell Rep Methods. 2022 May 25;2(6):100226. doi: 10.1016/j.crmeth.2022.100226. eCollection 2022 Jun 20. 10.1016/j.crmeth.2022.100226 PubMed 35784652
