Skip to main content
Addgene

pET15 PemrR-gfp biosensor
(Plasmid #185793)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185793 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 6829
  • Modifications to backbone
    A modified pET15b vector with a thrombin cleavage site and one of its his-tag removed. A TEV cleavage site is added.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pEmrR-gfp
  • Species
    Synthetic
  • Insert Size (bp)
    1121
  • Promoter PemrR
  • Tag / Fusion Protein
    • EGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SgrAI (unknown if destroyed)
  • 3′ cloning site BglIII (unknown if destroyed)
  • 5′ sequencing primer caccggcgactagtCGCAGCATTATCATCC
  • 3′ sequencing primer agatctgctagcTATAAACGCAGAAAGGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15 PemrR-gfp biosensor was a gift from Steven Hallam (Addgene plasmid # 185793 ; http://n2t.net/addgene:185793 ; RRID:Addgene_185793)

  • For your References section:

    An Improved Whole-Cell Biosensor for the Discovery of Lignin-Transforming Enzymes in Functional Metagenomic Screens. Ho JCH, Pawar SV, Hallam SJ, Yadav VG. ACS Synth Biol. 2018 Feb 16;7(2):392-398. doi: 10.1021/acssynbio.7b00412. Epub 2017 Dec 4. 10.1021/acssynbio.7b00412 PubMed 29182267
Related items: