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Addgene

pET28a-RBP-LOV2
(Plasmid #185792)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185792 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RBP-LOV2
  • Species
    Avena sativa
  • Promoter T7
  • Tag / Fusion Protein
    • 10xHis, TEV protease cleavage site after His Tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there is a A93C mutation in the insert. Depositor confirms this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-RBP-LOV2 was a gift from Lizhong He (Addgene plasmid # 185792 ; http://n2t.net/addgene:185792 ; RRID:Addgene_185792)

  • For your References section:

    Open-Closed Structure of Light-Responsive Protein LOV2 Regulates Its Molecular Interaction with a Binding Partner. Younas T, Vidallon MLP, Tabor RF, He L. J Phys Chem Lett. 2020 Oct 15;11(20):8647-8653. doi: 10.1021/acs.jpclett.0c02252. Epub 2020 Sep 29. 10.1021/acs.jpclett.0c02252 PubMed 32945680
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