pET28a-RBP-GFP(S45C)-ZdK1
(Plasmid
#185791)
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PurposeEncodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185791 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRBP-sfGFP(S45C)-ZdK1
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SpeciesAequorea victoria
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MutationSer to Cys mutation at start of GFP, residue 45 in frame
- Promoter T7
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Tag
/ Fusion Protein
- 10xHis, TEV protease cleavage site after His Tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-RBP-GFP(S45C)-ZdK1 was a gift from Lizhong He (Addgene plasmid # 185791 ; http://n2t.net/addgene:185791 ; RRID:Addgene_185791) -
For your References section:
Open-Closed Structure of Light-Responsive Protein LOV2 Regulates Its Molecular Interaction with a Binding Partner. Younas T, Vidallon MLP, Tabor RF, He L. J Phys Chem Lett. 2020 Oct 15;11(20):8647-8653. doi: 10.1021/acs.jpclett.0c02252. Epub 2020 Sep 29. 10.1021/acs.jpclett.0c02252 PubMed 32945680
