pET28a-ATA-P(11)4
(Plasmid
#185787)
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PurposeEncodes enzyme ω-aminotransferase linked to P(11)4 self-assembling peptide via a GS-linker sequence
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATA-P(11)4
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SpeciesAspergillus terreus
- Promoter T7
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Tag
/ Fusion Protein
- His-tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-ATA-P(11)4 was a gift from Lizhong He (Addgene plasmid # 185787 ; http://n2t.net/addgene:185787 ; RRID:Addgene_185787) -
For your References section:
Custom Design of Protein Particles as Multifunctional Biomaterials. Shanbhag BK, Liu C, Pradeep GC, Younas T, Hu KKY, Fulcher AJ, Struwe WB, Steer D, Dumsday G, Harper IS, Kukura P, Haritos VS, He L. Adv. Funct. Mater. 2022, 32, 2108039 10.1002/adfm.202108039